Publication by Mehmet Kerasu et al.: Removal of TREX1 activity enhances CRISPR-Cas9-mediated homologous recombination.

Karasu et al. discovered that TREX1, an exonuclease, suppresses CRISPR-Cas9-mediated HDR. Modifying DNA repair templates or reducing TREX1 expression before CRISPR genome editing significantly increases HDR efficiencies.