Sznurkowska et al. reveal that the liver promotes, while the lung restrains, secondary metastatic spread of breast cancer. The study identifies endothelial BMP2 as a key liver-derived signal that metabolically primes cancer cells for further issemination, highlighting a potential therapeutic target.
Vykhlyantseva et al. identified PARP1 as a context-dependent inhibitor of prime editing, showing that PARP1 inhibition enhances precise DNA insertions across different editing systems and cell types, including primary human T cells.
Using RaPID-seq, Yeh et al. showed that DNA homology-directed repair template search is constrained to proximal DNA sequences in humans, while exogenous genome-editing templates bypass these constraints and are efficiently utilized.
Gorbenko & Sala et al. engineered hyperactive compact CRISPR nucleases by directed evolution in human cells. Cas12f1Super and TnpBSuper show improved editing efficiency without increasing off-target activity, enabling AAV-based genome editing.