And here is the accompanying text: Vykhlyantseva et al. identified PARP1 as a context-dependent inhibitor of prime editing, showing that PARP1 inhibition enhances precise DNA insertions across different editing systems and cell types, including primary human T cells.
Using RaPID-seq, Yeh et al. showed that DNA homology-directed repair template search is constrained to proximal DNA sequences in humans, while exogenous genome-editing templates bypass these constraints and are efficiently utilized.
Gorbenko & Sala et al. engineered hyperactive compact CRISPR nucleases by directed evolution in human cells. Cas12f1Super and TnpBSuper show improved editing efficiency without increasing off-target activity, enabling AAV-based genome editing.
Silva & Mayrhofer et al. show that during chronic metabolic stress Tent5a expression is increased, leading to elongation of poly(A) tails, stabilization of the insulin mRNA, and increasing insulin synthesis. This helps augment insulin secretion during increased metabolic demand.