Publication by Aizawa et al.: Haploid mouse germ cell precursors from embryonic stem cells reveal Xist activation from a single X chromosome.

Here, we characterize a culture system for obtaining haploid primordial germ cell-like cells (PGCLCs) from haploid mouse embryonic stem cells (ESCs). We find that haploid cells show predisposition for PGCLCs over somatic cells. Moreover, a single X chromosome in haploid cells leads to activation of Xist, and mutation of Xist is insufficient to prevent diploidization of haESCs.